¾­µäÐźÅͨ·¹ãɸPCRоƬ(Signaling Pathway Profiling PCR Array-1, SPP-1) Ñ¡È¡ÁË15Ìõ¾­µäͨ·£¨MAPK¡¢mTOR¡¢JAK-STATµÈ£©ÖеÄ168¸ö½Úµã»ùÒò½øÐÐÕûºÏ¼ì²â¡£ÕâЩÐźÅͨ·¹ã·º²ÎÓëϸ°ûµÄÔöÖ³¡¢·Ö»¯¡¢µòÍö¡¢´Ù½øÎïÖÊ´úл¡¢ÒÔ¼°ÃâÒßµ÷½ÚµÈÐí¶àÖØÒªµÄÉúÎïѧ¹ý³Ì¡£SPP1оƬ¿Éͨ¹ýÒ»´ÎʵÑéÍê³É¶àÌõͨ·½Úµã»ùÒòµÄ¼ì²â£¬²»½öÓÐÀûÓÚ¿ìËÙ·¢ÏÖʵÑéÑù±¾ÖйؼüµÄµ÷±äͨ·£¬¶øÇÒÓÐÖúÓÚÀí½âÕâЩͨ·¼äµÄÍøÂçµ÷¿Ø¹Øϵ£¨cross-talk£©¡£Í¬Ê±£¬PCRоƬ½á¹û²»ÐèÒªÔÙ½øÐÐÑéÖ¤£¬¶ÔÓÚÑùÆ·¡¢Ê±¼ä¡¢¾­·ÑÒ²¸ü¼Ó½ÚÊ¡¡£

 

 

 

    |   Í¨Â·£¨15Ìõ£©ºÍ»ùÒò£¨168¸ö£©

¡ñ  mTOR Signaling Pathway£ºAKT£»AKT1S1£»EIF4B£»EIF4E£»EIF4EBP1£»IKBKB£»IRS1£»MTOR£»MTOR£»PDPK1£»PIK3R1£»PRKAA1£»PRKCA£»PTEN£»RPS6£»RPS6KA1£»RPS6KB1£»RPS6KB1£»STK11

¡ñ  JAK / STAT Signaling Pathway: AKT3£»BCL2L1£»CCND1£»EP300£»JAK1£»MYC£»PTPN11£»PTPN6£»STAT1

¡ñ  MAP Kinase Signaling Pathway: AKT3£»ARRB1£»ATF4£»BRAF£»CASP3£»DAXX£»DUSP1£»ELK1£»FAS£»FLNA£»HSPB1£»IKBKG£»JUN£»JUND£»MAP2K1£»MAP2K2£»MAP2K3£»MAP2K4£»MAP2K6£»MAP2K7£»MAP3K1£»MAP3K5£»MAP3K7£»MAP3K8£»MAPK14£»MAPKAPK2£»MYC£»NFATC3£»PAK1£»PRKCB£»RAC1£»RAF1£»RASGRF1£»RELB£»RPS6KA1£»STMN1£»TGFBR1

¡ñ  NFkB Signaling Pathway: BCL2£»BCL2L1£»BTK£»CHUK£»IKBKB£»IKBKG£»MAP3K7£»NFKB1£»NFKB2£»NFKBIA£»RELA

¡ñ  Notch Signaling Pathway: ADAM17£»DLL3£»HDAC1£»HES1£»HEY1£»JAG1£»LFNG£»NCOR2£»NCSTN£»NOTCH1£»NUMB£»PSEN1£»PSENEN£»PTCRA£»RBPJL£»SNW1

¡ñ  PI3K-AKT Signaling Pathway: AKT3£»BAD£»BCL2£»BCL2L1£»BCL2L11£»CCND1£»CDK2£»CDKN1A£»CDKN1B£»CHUK£»CSF1R£»EIF4B£»EIF4E£»EIF4EBP1£»FOXO3£»GSK3B£»IFNAR1£»IRS1£»JAK1£»MAP2K1£»MDM2£»MTOR£»MYC£»NFKB1£»PDPK1£»PRKCA£»PTEN£»PTK2£»RAF1£»RBL2£»RPS6£»RPS6KB1£»TP53£»TSC2£»YWHAQ

¡ñ  Apoptosis Signaling Pathway: AKT3£»BAD£»BAX£»BCL2£»BCL2L1£»BID£»CASP3£»CASP6£»CASP8£»CASP9£»CHUK£»FADD£»FAS£»NFKB1£»NFKBIA£»TP53£»XIAP

¡ñ  Cell Cycle Signaling Pathway: ABL1£»CCNB1£»CCND1£»CCNE1£»CDC25A£»CDC25B£»CDK1£»CDK2£»CDKN1A£»CDKN1B£»CHEK1£»E2F1£»GSK3B£»HDAC1£»MDM2£»MYC£»PLK1£»PRKDC£»RB1£»RBL2£»SMAD2£»TP53£»WEE1£»YWHAQ

¡ñ  VEGF Signaling Pathway: AKT3£»CASP9£»HSPB1£»MAP2K1£»MAPK14£»NOS3£»PLCG1£»PRKCA£»PTK2£»PXN£»RAC1£»RAF1

¡ñ  TGFß Signaling Pathway: MYC£»RPS6KB1£»SMAD1£»SMAD2£»SP1£»TGFBR1£»TGFBR2

¡ñ  Hippo Signaling Pathway£º AMOT£»FBXW11£»FRMD6£»LATS2£»MOB1A£»NF2£»PPP1CA£»PPP2CA£»SAV1£»SMAD2£»SMAD4£»SMAD7£»TP53BP2£»YAP1

¡ñ  Epithelial to Mesenchymal Transition (EMT): AKT3£»CTNNB1£»EGFR£»FGFR1£»FN1£»GSK3B£»MSN£»PTK2£»RAC1£»STAT3£»TGFB1

¡ñ  Autophagy: AKT1S1£»AKT3£»ATG10£»ATG12£»ATG13£»ATG3£»ATG5£»ATG7£»BAD£»BCL2£»BNIP3£»IGF1R£»IRS1£»MAP3K7£»MTOR£»PTEN£»RAF1

¡ñ  Cancer Drug Resistance: AKT3£»ATM£»BAX£»BCL2£»BCL2L1£»BRCA1£»CCND1£»CCNE1£»CDK2£»CDK4£»CDKN1A£»CDKN1B£»CDKN2A£»EGFR£»ERBB2£»FOS£»IGF1R£»NFKB1£»PPARD£»RXRA£»TP53

¡ñ  Cancer Drug Targets: AKT3£»BCL2£»CDC25A£»EGFR£»GRB2£»HRAS£»HSP90AA1£»IGF1£»IGF1R£»IGF2£»KIT£»KRAS£»MDM2£»MTOR£»NFKB1£»PDGFRA£»PDGFRB£»PIK3CA£»PRKCA£»RHOA£»TP53

|   PCRоƬʵÑéÁ÷³Ì

¡ñ   RNAÖƱ¸´¿»¯²¢Öʼ죨²»·ûºÏÒªÇóµÄÐèÒªÖØÐÂÌṩÑùÆ·Ëͼ죩

¡ñ   RNA·´×ªÂ¼ÎªcDNA

¡ñ   ÅäÖ÷´Ó¦Ìåϵ·ÅÈë¹Ì¶¨ºÃ»ùÒòÌØÒìÐÔÒýÎïµÄ96¿×°å¸÷¿×ÖÐ

¡ñ   ½«°åÖÃÓÚPCRÒÇÖУ¬ÔËÐгÌÐò

¡ñ   ¼ÆËãÿ¸ö»ùÒòµÄÑ­»·ãÐÖµ(Ct)·ÖÎö

¡ñ   ³ö¾ßʵÑ鱨¸æ°üÀ¨ÊµÑé·½·¨¼°ÊµÊ±¶¨Á¿PCRоƬʵÑé½á¹û¼°Ïà¹Øͼ±í

|   ½á¹ûչʾ

|   PCRоƬ³£¼ûÎÊÌâ

Q1¡¢PCR оƬµÄÖÊÁ¿ÈçºÎ£¿

ʵÑéÖеÄÒýÎÊÔ¼Á£¬ÊµÑéÌõ¼þ£¬²Ù×÷Á÷³Ì¶¼¾­¹ýÑϸñµØÖʼì¡£

Q2¡¢PCR оƬΪʲôѡÓöà¸ö¿´¼Ò»ùÒòµÄ×÷Óã¿

Ñо¿·¢ÏÖ²¢²»ÊÇËùÓеĿ´¼Ò»ùÒòÔÚϸ°ûºÍ×éÖ¯µÄËùÓн׶ξùºã¶¨±í´ï£¬Èç¹ûֻѡÓÃ1-2¸ö¿´¼Ò»ùÒò×÷ΪÄڲΣ¬¿ÉÄÜ»áµÃµ½´íÎóµÄ¿Æѧ½áÂÛ¡£

Q3¡¢PCR оƬÊÇ·ñÐèÒª½øÐм¼ÊõÖظ´ÊµÑ飿

ÍƼöÉúÎïѧÖظ´£¬ÒòΪPCR ArrayµÄÖظ´ÐԷdz£ºÃ£¬ÎÞÐè¼¼ÊõÖظ´¡£

Q4¡¢PCRоƬʵÑéµÄRNA ÓÃÁ¿£¿

ÿ¿é96 ¿×°åÉÏÒ»¸öÑù±¾1000ng ×óÓÒµÄ×ÜRNA£¬

Q5¡¢PCR оƬÉϵĻùÒòÊÇÔõôѡÔñµÄ£¿

ÕâЩ±»Ñ¡ÖеĻùÒò±ØÐë¶ÔÓÚÏàÓ¦µÄÐźÅתµ¼Í¾¾¶Ï൱ÖØÒª£¬²¢ÇÒÊÇÔÚmRNA ˮƽÉϵ÷¿ØµÄ¡£Ñз¢ÈËÔ±¼°ÉúÎïÐÅϢѧ¿Æѧ¼Òͨ¹ýÔĶÁÒÑ·¢±íÎÄÏ×»ò¶à¸öÊý¾Ý¿âÀ´É¸Ñ¡Ïà¹ØµÄ»ùÒò£¬È»ºóÔÙÓÃÎı¾·¢¾òµÄ·½·¨½øÐÐУ¶Ô¡£