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[1]. Wang Feng, Qian Jin, Yang Ming-Yu, et al. MiR-6924-5p-rich exosomes derived from genetically modified Scleraxis-overexpressing PDGFRα(+) BMMSCs as novel nanotherapeutics for treating osteolysis during tendon-bone healing and improving healing strength. Biomaterials, 2021 Dec;279:121242. IF=12.479

[2]. Dong Z ,  Gu H ,  Guo Q , et al. Profiling of Serum Exosome MiRNA Reveals the Potential of a MiRNA Panel as Diagnostic Biomarker for Alzheimer's Disease[J]. Molecular Neurobiology, 2021(428-445). IF= 5.590

[3]. Bai X, Li J, Li L, et al. Extracellular Vesicles From Adipose Tissue-Derived Stem Cells Affect Notch-miR148a-3p Axis to Regulate Polarization of Macrophages and Alleviate Sepsis in Mice[J]. Frontiers in immunology, 2020, 11: 1391. IF= 7.561

[4]. Bian X, Li B, Yang J, et al. Regenerative and protective effects of dMSC-sEVs on high-glucose-induced senescent fibroblasts by suppressing RAGE pathway and activating Smad pathway[J]. Stem Cell Research & Therapy, 2020, 11: 1-16. IF= 5.985

[5]. Qiao L, Hu S, Liu S, et al. microRNA-21-5p dysregulation in exosomes derived from heart failure patients impairs regenerative potential[J]. The Journal of clinical investigation, 2019, 129(6): 2237-2250. IF=14.808